Journal:

Article Title: Invasiveness of Hepatocellular Carcinoma Cell Lines: Contribution of Membrane-Type 1 Matrix Metalloproteinase 1

doi:

Figure Lengend Snippet: Induction of the expression of MT1-MMP by HGF in JHH-7 cells. (A), RT-PCR was performed with total RNA isolated from the JHH-7 cells, which had been treated with HGF (10 ng/ml) for 24 hours. Thirty-six and 21 amplification cycles were used with human MT1-MMP and GAPDH primers, respectively. The abundance of MT1-MMP relative to that of GAPDH was determined by using a Master Scan Gel Analysis System. Flow-cytometric analysis was performed using untreated (B) and HGF-treated (C) JHH-7 cells. The cells were incubated with anti-MT1-MMP rabbit antibody followed by FITC-conjugated anti-rabbit IgG to analyze the fluorescense intensity.

Article Snippet: The cells were harvested and incubated with rabbit anti-MT-MMP (CHEMICON International Inc., CA) antibody followed by FITC-conjugated goat anti-rabbit IgG antibody (Cedarlane Lab. Ltd., Ontario, Canada).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation, Amplification, Incubation

Journal: PLoS ONE

Article Title: Differential Effects of Serum Heat Treatment on Chemotaxis and Phagocytosis by Human Neutrophils

doi: 10.1371/journal.pone.0054735

Figure Lengend Snippet: ( A ) Results of staining with FITC-conjugated anti-C3b. ( B ) Results of staining with Alexa-Fluor®-488-conjugated anti-IgG. In all cases, the particles had been incubated in three different buffers, i.e., serum-free HBSS (“plain”), HBSS with 10% untreated autologous serum (37°C-ASPO), and HBSS with 10% autologous serum that had been heat-treated at 56°C (56°C-ASPO). The legend included in (A) applies to all panels.

Article Snippet: Opsonized and plain zymosan particles were incubated with 1 µg/mL FITC-conjugated mouse anti-human C3b (Accurate Chemical & Scientific Corp., Westbury, NY, USA) or Alexa-Fluor®-488-conjugated goat anti-human IgG (Invitrogen) monoclonal antibodies at room temperature on a rotator for 30 min, and washed twice.

Techniques: Staining, Incubation

Journal: PLoS ONE

Article Title: Differential Effects of Serum Heat Treatment on Chemotaxis and Phagocytosis by Human Neutrophils

doi: 10.1371/journal.pone.0054735

Figure Lengend Snippet: ( A ) Results of staining with FITC-conjugated anti-C3b. ( B ) Results of staining with Alexa-Fluor®-488-conjugated anti-IgG. The first three columns depict example data obtained with sera from different, healthy donors. The rightmost column summarizes the results for a total of 3 donors. Values are block-centered means ± SD.

Article Snippet: Opsonized and plain zymosan particles were incubated with 1 µg/mL FITC-conjugated mouse anti-human C3b (Accurate Chemical & Scientific Corp., Westbury, NY, USA) or Alexa-Fluor®-488-conjugated goat anti-human IgG (Invitrogen) monoclonal antibodies at room temperature on a rotator for 30 min, and washed twice.

Techniques: Staining, Blocking Assay